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International Journal of Applied Research
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ISSN Print: 2394-7500, ISSN Online: 2394-5869, CODEN: IJARPF

Impact Factor: RJIF 5.2

International Journal of Applied Research

Vol. 2, Issue 12, Part A (2016)

To study intracellular bactericidal activity of Isoniazid & Rifampicin, alone and in combination with Isoxyl against standard and multi drug resistant strain of Mycobacterium tuberculosis in macrophage cell line

Author(s)
Shashikant Vaidya, Shreyasi Muley, Rashmi Shimpi, Mohan Kulkarni, Geeta Koppikar and Abhay Chowdhary
Abstract
Tuberculosis (TB) remains a major health problem in developing nations. As drug development is a long and expensive process, it becomes predominant to reexamine drugs that were formerly deemed effective against TB and increase the permeability of the Mycobacterial cell wall. One such drug is Isoxyl (ISO). Interest in the use of combination of antibiotics was started many years ago, almost as soon as two antibiotics were available. To minimize the probability of emergence of drug resistance, to increase the activity, particularly the bactericidal activity of the agents. Current use of drug combinations in the therapy of TB is aimed at designing the most efficient short-course regimens. It is reported the use of a murine macrophage continuous cell line J774 as a model for testing the susceptibility of mycobacteria to antimicrobial agents. Present study was conducted with the objective of determining the efficacy of ISO. To assess cytotoxic reaction to macrophage cell line by various concentrations of ISO. To study intracellular bactericidal activity of ISO against standard strain of Mycobacterium tuberculosis H37Rv in macrophage cell line. To study intracellular bactericidal activity of INH and RF, alone and in combination with ISO against standard and MDR strain of M. tuberculosis in macrophage cell line. It is observed that, this thiourea is apparently not acutely toxic for macrophage cell line J 774A.1. It showed some bactericidal activity in intracellular bacteria. There is an intracellular synergistic interaction, though little, between ISO & INH and ISO & RF against standard strain and MDR strain of M. tuberculosis
Pages: 27-32  |  643 Views  18 Downloads
How to cite this article:
Shashikant Vaidya, Shreyasi Muley, Rashmi Shimpi, Mohan Kulkarni, Geeta Koppikar and Abhay Chowdhary. To study intracellular bactericidal activity of Isoniazid & Rifampicin, alone and in combination with Isoxyl against standard and multi drug resistant strain of Mycobacterium tuberculosis in macrophage cell line. International Journal of Applied Research. 2016; 2(12): 27-32.
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