Vol. 2, Issue 6, Part G (2016)

Abstract
The liver is the only visceral organ that possesses remarkable capacity to regenerate. The liver can regenerate after either surgical removal or after chemical injury. It is known that as little as 25% of the original liver mass can regenerate back to its full size. The process of regeneration in mammals is mainly compensatory growth because only the mass of the liver is replaced not the shape. Liver regeneration involves replication of the liver cells, mainly hepatocytes, followed by other cells such as biliary epithelial cells and sinusoidal endothelial cells. Once cell proliferation is completed, the newly divided cells undergo restructuring, angiogenesis and reformation of extracellular matrix to complete the regeneration process. The main objective of present research work is to screen the bio molecules present in EE-CS and isolate the bioactive carotenoid rodoxanthine and evaluate the in vivo hepatocytes regenerators’ potentiality. Characterization of rodoxanthine was carried out by U.V, IR, ¹H-NMR, ¹³C-NMR, Mass spectra and HPLC etc. The in vivo hepatocytes regenerator’s potentiality was performed against CCl₄ induced rat hepatocytes. The results obtained from the in vivo experimental data had shown that the elevated levels of SGOT, SGPT, ALP and Serum bilirubin due to CCl₄ intoxication were reduced significantly (*P<0.05) in rats, after treatment with EE-CS. Treatment with EE-CS at a both doses of 250 and 500 mg/kg b. w. significantly decreased the SGOT, SGPT, ALP, serum bilirubin levels by 13.05%, 27.98%, 8.82%, and 16.23% (at low dose) and 26.1%, 47.16%, 24.34% and 43.58% (at high dose) respectively. Silymarin used as standard drug showed a reduction of 55.09%, 68.98%, 57.46% and 60.68% receiving CCl₄ alone. So depending upon the recent data it was confirmed that the biochemical parameters of the group treated with EE-CS was significantly lower than the CCl₄-treated group. Histopathological analysis of rat liver sections obtained from CCl₄-intoxicated rats had shown a variety of cavitations and necrosis in hepatocytes, liver tissue section prepared from the 250 mg/kg EE-CS-treated group displayed less cavitation and necrosis, liver tissue section prepared from the 500 mg/kg EE-CS-treated group displayed less cavitation and necrosis, and liver tissue section prepared from the std. drug silymarin-treated group had shown centrilobular regeneration with restoration of central vein, sinusoids and hepatocytes with mild necrosis.