International Journal of Applied Research
Vol. 2, Issue 6, Part G (2016)
Studies of melanin producing bacteria and extraction of bacterial melanin from sewage water
AN Pathan and AS Pethe
Melanin are the natural pigments which have their presence in animals, plants and in most of the microorganisms. The current study is aimed to isolate and identify melanin producing bacteria. The sewage water samples were randomly collected from two district Akola and Buldhana.
A nutrient agar medium containing tyrosine was used for isolation of melanin producing bacteria, which were examined for biochemical and morphological test. Isolates were then subculture on nutrient agar medium containing tyrosine for production of gas from glucose, growth in presence of Nacl, Pᴴ tolerance, growth temperature and enzymatic activity. The melanin pigment was further characterized by FT-IR spectroscopy. The FT-IR result confirms that it was melanin pigment. Therefore, this study proved that sewage water sample can be used for the production of melanin and it has resistant anti-bacterial activity. In present study different bacterial species of Azotobacter spp. Pseudomonas spp. and Bacillus spp. were identified.
How to cite this article:
AN Pathan and AS Pethe. Studies of melanin producing bacteria and extraction of bacterial melanin from sewage water. International Journal of Applied Research. 2016; 2(6): 413-415.