International Journal of Applied Research
Vol. 3, Issue 5, Part H (2017)
Hepatic Biotransformation of Chlordecone and Induction of Hepatotoxicity in the Cichlid Fish, Pseudetroplus maculatus (Bloch, 1795)
KP Asifa and KC Chitra
Detoxification mechanism in hepatic tissue after exposure to one of the environmental contaminants, chlordecone, was investigated by analysing certain biotransformation biomarkers of phase I enzyme - hepatic 7-ethoxyresorufin-O-deethylase (EROD), and Phase II enzymes - UDP-glucuronosyltransferase (UGT), glutathione-S-transferase (GST) and gamma-glutamyl transpeptidase (GGT) in the cytosolic and microsomal fractions of liver tissue in the cichlid fish, Pseudetroplus maculatus. Two sublethal concentrations of chlordecone (3.5 and 7µg/L) were exposed to fish for short durations as 24, 72 and 96 h. At the end of treatment period, the activities of EROD, UGT and GGT were increased significantly (P<0.05) in concentration and time-dependent manner when compared to the control groups. The present observation clearly illustrates that chlordecone promoted the activities of biotransformation enzymes in liver in order to eliminate the toxic compound from the body of the animal. The activity of GST was decreased significantly (P<0.05) after chlordecone exposure, which reflects the inability of liver tissue to neutralize the oxygen free radicals generated. Thus the obtained data contribute to a better understanding of chlordecone-induced detoxification mechanism in liver of fish, which further resulted in hepatotoxicity in the cichlid fish, Pseudetroplus maculatus
How to cite this article:
KP Asifa and KC Chitra. Hepatic Biotransformation of Chlordecone and Induction of Hepatotoxicity in the Cichlid Fish, Pseudetroplus maculatus (Bloch, 1795). International Journal of Applied Research. 2017; 3(5): 521-526.