Introduction: Blood has always been considered essential for the maintenance of life. The preservation of human red blood cells (RBCs) outside the body is a rather remarkable sphenomenon when you think about it. The aim of this study is to measure the trend in the levels of haematological and biochemical parameters in whole blood from the day of donation till the last day of acceptable storage. This will eventually highlight the potentials and limitations of stored whole blood as a source of leucocytes and/or platelets in transfusion therapy for patients with leucopenia and/ or thrombocytopenia.
Material and Method: This is a prospective study carried out in the Department of Pathology, S.S. Medical College Rewa on 50 healthy voluntary donors included were those who donated blood in the blood bank of Sanjay Gandhi Memorial Hospital, Rewa (M.P). Haematological parameters were measured in each donor’s blood bag immediately and on 7th, 14th, 21st 28th and 35th day intervals using auto analyser.
Result: The mean values of Red cell count showing no significant changes. By statistical analysis ANOVA (F- test) P<0.1472 and it is non significant. In present study, mean hemoglobin levels on day one was 11.36 gm/dl gradually falling till the last storage day up to 10.7 gm/dl showing only slight reduction. The mean values of MCV were on day one is 82.19 fl, while progressively becoming 122.46 fl on day 35th. By statistical analysis ANOVA (F- test) P< 0.0001 and it is highly significant. The mean values of HCT showing slight reduction only during storage, However by statistical analysis ANOVA (F- Test) P<0.004 and it is significant. The mean values of WBC on day 1 were compared to other storage day. It was observed that there was rapid deterioration in WBC count. By statistical analysis ANOVA (F-test) P<0.0001 and it is highly significant. The mean values of granulocyte count on day one were compared to other storage day. It was observed that there was rapid deterioration in granulocyte. By statistical analysis ANOVA (F- test) P< 0.0001 and it is highly significant. The mean values of lymphocyte reduced during storage. By statistical analysis ANOVA (F-test) P<0.0001 and it is highly significant.
Conclusion: From the present study it is concluded that, CPDA-1 whole blood can be used up to the last acceptable storage day that is 35th day in case of low RBC count. During storage, we observed that there was rapid deterioration in leucocyte count. Postoperative risk of bacterial infection increases in patient having major surgery so fresh blood (less than 7 days) indicated. In thrombocytopenia patients, we should use fresh whole blood as far as possible (less than 7 days) and platelet transfusion should be preferred over whole blood.