International Journal of Applied Research
Vol. 5, Issue 2, Part B (2019)
Evaluation of germ tube test in various media
Dr. Nasira Shaikh, Dr. Sapana Mundhada, Dr. Vilas Jahagirdar and Dr. Kishor Ingole
Systemic candidiasis is responsible for a high mortality rate, even after antifungal therapy. Candida albicans is the main cause of invasive fungal diseases which is a serious public health issue. Various morphological, biochemical, and molecular methods are available for the identification of C. albicans still Germ tube test in a pooled human serum remain the test of choice. It is simple, rapid, and highly reliable test that has been used for many years but handling human serum during test is associated with acquisition of dangerous infection like hepatitis and HIV. In present study we had evaluated germ tube test in various media like 1% nutrient broth, 1% peptone water, 0.5% glucose broth, Glycine (50mg per dL in 0.5% glucose broth), Histidine (50 mg per dL in 0.5% glucose broth) Cystine (50 mg per dL in 0.5% glucose broth) for comparison with recommended standard germ tube testing in human serum.50 strain of Candida albicans isolated from 350 samples were subjected to various media for studying production of germ tube. In 98% cases pooled human serum remain the best media. It was observed that, When 72 hour old cultures were used for preparation of test inoculate and Candida strain present in it starved for more than 60 minutes chances of germ tube positivity was increased.
How to cite this article:
Dr. Nasira Shaikh, Dr. Sapana Mundhada, Dr. Vilas Jahagirdar and Dr. Kishor Ingole. Evaluation of germ tube test in various media. International Journal of Applied Research. 2019; 5(2): 114-117.