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International Journal of Applied Research
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ISSN Print: 2394-7500, ISSN Online: 2394-5869, CODEN: IJARPF

IMPACT FACTOR (RJIF): 8.4

Vol. 6, Issue 7, Part G (2020)

Protective effects of aqueous and ethanol extracts of Ficus religiosa Leaves and Bark on H2O2-induced oxidative DNA damage in human lymphocytes by comet assay

Protective effects of aqueous and ethanol extracts of Ficus religiosa Leaves and Bark on H2O2-induced oxidative DNA damage in human lymphocytes by comet assay

Author(s)
Dr. Sabitha Y and BSR Kumari
Abstract
Ficus religiosa is traditionally used as medice to treat various diseases which include Otitis media, Mastitis, Diabetis, Pharyngolaryngitis, Dysmenorrhea and Urethritis. The present study deals with the protective effects of the aqueous and the ethanol extracts of Ficus religiosa leaves and bark on H2O2 –induced oxidative DNA damage in human lymphocytes by the comet assay.
Fresh leaves and bark of Ficus religiosa were collected from Draksharam, Bhimeswara swamy temple to perform antioxidation and comet assay. The leaves and bark were washed under running tap water, air dried and powdered finely and used for further studies.
The results showed that the tail lengths are increased when the samples are treated with the hydrogen peroxide. Increased tail length denotes that the more damage is occurred to the genetic material and the damage is increasing along with the increase in the concentration of hydrogen peroxide.
Among the four extracts of Ficus religiosa (Leaf Ethanolic extract, Leaf aqueous extract, Bark Ethanolic extract, bark aqueous extract) leaf Ethanolic extract has more antigenotoxic activity because, the inhibition percentage of the Leaf Ethanolic extract and the p value of this is statistically very significant. The nature of antigenotoxicity is increasing with the increasing concentration. Even at the low concentration of this extract, significant inhibition rate is observed.
Pages: 480-486  |  792 Views  120 Downloads
How to cite this article:
Dr. Sabitha Y, BSR Kumari. Protective effects of aqueous and ethanol extracts of Ficus religiosa Leaves and Bark on H2O2-induced oxidative DNA damage in human lymphocytes by comet assay. Int J Appl Res 2020;6(7):480-486.
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