Vol. 1, Issue 11, Part P (2015)

Material and method: Endoscopy was performed with Fujinon-fibre-scope UGl-FP-7 gastroduodenoscope after obtaining informed consent. Endoscopy was done as one of the routine investigation procedure and the samples were sent to the microbiology department for culture and smear examination.

Results: Out of 100 biopsies from symptomatic patients which were subjected to modified Gram’s staining H. pylori was demonstrated in 52 patients giving percentage positivity of 52 %. Highest positivity was seen in patients with gastritis and duodenitis. Smear was negative for H. pylori in all 10 controls. Fontana staining was also done on all biopsy samples. It appears that spiral morphology is better appreciated in Fontana stainig as compared to modified Gram’s staining.

Conclusion: Rapid urease test appears to be more useful for rapid detection of H.pylori than smear and culture. Fontana's staining although more expensive and time consuming than modified Gram's stain, it stains bacteria well which can be easily detected and spiral morphology of H.pylori is better appreciated.