International Journal of Applied Research
Vol. 1, Issue 13, Part L (2015)
Anti-mycobacterial activity of garlic (Allium sativum) against multi-drug resistant and reference strain of Mycobacterium tuberculosis
Smita D Rajani, Pratibha B Desai, Dhanji P Rajani
Emergence of multi-drug resistant (MDR) and extensively-drug resistant (XDR) strains of Mycobacterium tuberculosis has further complicated the problem of tuberculosis (TB) control. There is a basic need to look at alternative anti-TB agents. Medicinal plants present an anticipation for developing alternate medicines for the treatment of TB. Garlic (Allium sativum) is one of the natural plant which possesses variety of biological properties like anti-tumor, anti-hyperlipidemic and anti-microbial etc. The present study was completed to evaluate in vitro anti-tubercular activity of garlic against MDR, XDR and reference strain of M. tuberculosis H37Rv. Ethanolic extract of garlic was set by maceration method. Minimum inhibitory concentration (MIC) was performed by using 7H9 middle brook broth dilution technique on 48 MDR isolates out of total 230 clinical isolates of MTB and reference strain of M. tuberculosis H37Rv. MIC of garlic extract was ranged from 0.5 to 2 mg/ml, showing inhibitory effects of garlic against both MDR and XDR M. tuberculosis isolates. Alternate medicine practices with plant extracts including garlic should be considered to decrease the burden of drug resistance and cost in the management of diseases. The use of garlic against MDR-TB may be of great significance regarding to public health.
How to cite this article:
Smita D Rajani, Pratibha B Desai, Dhanji P Rajani. Anti-mycobacterial activity of garlic (Allium sativum) against multi-drug resistant and reference strain of Mycobacterium tuberculosis. Int J Appl Res 2015;1(13):767-770.