Vol. 1, Issue 9, Part L (2015)

Abstract
A study was conducted to adaptation of mammalian cell from 10% serum medium to to serum free or low serum media, to subcultured cell line Baby hamster Kidney-21 (BHK-21) to optimize the In vitro culture requirement and conditions for maintenance and long time cryopreservation. BHK-21 cells multiply fast during first 48 hrs and make a complete layer and got a confluence within 72 hrs post incubation, followed by a decline phase. Fetal bovine serum has a growth stimulating effect 10% to 3% serum level is satisfactory for the maintenance of the cell line. While harvesting the cells from a flask, Trypsin (0.25%) with neutralization by fetal bovine serum was found suitable. For cell storage 10% Dimethyl sulfoxide (DMSO) used to maintain maximum recovery of viable cells during cyropreservation.