This study also includes estimation of Cotinine, a metabolite of Nicotine in maternal blood using ELISA technique, which was not yet studied so far with respect to smokeless Tobacco.
Material and Method: Detailed history was taken from the subjects & from the case records as per the Proforma (Annexure I) on the day of delivery, which included personal details, diet history, obstetric history (present & past), history of parturition, anthropometric measurements of new born baby among users and non-users of Mishri. The detail of duration, frequency & amount of use as well as duration for which the Mishri was kept in mouth was collected . Maternal blood (1ml) of study subjects and controls were collected in a plain bulb within 24hours of delivery and was processed in Biochemistry laboratory of Krishna Institute, which included centrifugation, collection of serum in pendrop (plastic bulb), estimation of cotinine in serum by using ELISA kit method and then the levels of cotinine in serum were recorded in the respective proforma of respective participants (study subjects & controls) in nanogram per millimeter (ng/ml). All the recorded data of users & nonusers of smokeless tobacco (Mishri) were entered into the Microsoft excel sheet and appropriate statistical tests were applied to analyze the final data under guidance of the Statistician & Postgraduate Guide.
Result: Among users of Mishri no significant difference in mean levels of iron in diet was found when compared between those having Anemia & not having Anemia. Also no significant correlation was seen between hemoglobin levels and dietary iron of subjects using Mishri (r=0.047, p=4.53) whereas a significant correlation was seen among nonusers of Mishri (r=0.129, p=0.05).
Conclusion: With increasing duration it appears more and more tobacco (Mishri) is being consumed resulting in higher Cotinine levels and the adverse effects of tobacco on pregnant women and baby are accentuated.