International Journal of Applied Research
Vol. 2, Issue 11, Part D (2016)
Bioremediation of chromium by unique chromium reductase activity in Proteus sp. isolated from waste water
This is the first time where Proteus sp. has been isolated from waste water which has higher potential for bioremediation. Chromium (Cr) compounds are used in dyes and paints and in the tanning of leather. So they are found in soil and ground water at abundant industrial sites, now needing environmental clean-up and remediation. More toxic Cr(VI) is reduced by the chromium reducing bacteria to Cr(III) which is less toxic. Isolation of chromium reducing bacteria from water samples from East Kolkata Wetland leather complex was done to observe the effect of Chromium on them, to study their growth curve characteristics and for remediation assay of heavy metal contaminated industrial wastes by evaluating their Cr(VI) reducing ability through chromate reductase activity. Potassium dichromate (K2Cr2O7) and potassium chromate (K2CrO2) were employed for the growth of these bacteria. Attempts were made to isolate the genomic DNA of the organism and to amplify its 16S rRNA gene for identification of the organism using Bioinformatics tools. The organism identified was Proteus sp. Fourier transform infrared (FT-IR) spectroscopic study was performed to obtain information of the possible cell-metal ion interaction. This study has application in terms of bioremediation of metal contaminated industrial waste water with the bacteria isolated from waste water. This process is environment friendly and cost effective. Moreover the chromate reductase enzyme from isolated bacteria can be purified and immobilized which can be further used to detoxify of Cr from tannery wastes.
How to cite this article:
Puja Jhunjhunwala, Rini Roy, Santanu Roy, Aditi Nag Chaudhuri. Bioremediation of chromium by unique chromium reductase activity in Proteus sp. isolated from waste water. Int J Appl Res 2016;2(11):249-256.