International Journal of Applied Research
Vol. 2, Issue 3, Part F (2016)
Textile dye degradation using bacterial strains isolated from textile mill effluent
Textile industry generates waste water with a complex mixture of many pollutants such as heavy metals, chlorinated compounds, pigments and dyes. The presence of textile dyes even in low concentration in effluent is highly visible, undesirable and recalcitrant in the natural environment. After release into the water bodies these dyes have negative impact on photosynthesis of aquatic plants. Bacterial strains capable of degrading textile dyes were isolated from effluents of hand looms. The soil sample from Kannan hand loomps, Balaramapuram was used for the isolation of textile dye degrading organisms. Totally five bacterial strains were isolated and designated as TDP1, TDP2, TDG1, TDG2 and TDG3. The isolates were grown on nutrient agar plates with a textile dyes Provisional pink and Green PB (50mg/100ml). The morphological and biochemical characteristics of isolates were determined. The decolourising ability of the strains were detected using nutrient broth incorporated with dye and the OD reading was recorded by UV- Spectrophotometer at 592nm. More than 90% of Provisional pink was reduced and colour changes occurs within five days at a dye concentration of 50mg/l. The decolourising ability of the strains were detected using nutrient broth with different dye concentration (50mg, 100mg. 150mg, 200mg & 250mg) at different time intervals. All the five strains showed decolourising ability when incorporated in nutrient broth with dye. Among those strains TDP1 and TDG3 showed maximum ability to decolourise the textile dyes. Identification of the strain TDP1 and TDG3 was achieved by 16srRNA sequencing and was identified as Bacillus and Aeromonas hydrophila sp.
How to cite this article:
M Parimala Celia, S Suruthi. Textile dye degradation using bacterial strains isolated from textile mill effluent. Int J Appl Res 2016;2(3):337-341.