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ISSN Print: 2394-7500, ISSN Online: 2394-5869, CODEN: IJARPF
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Vol. 2, Issue 5, Part E (2016)
Evaluation of different time courses of bleomycin to induced interstitial pulmonary fibrosis in C57BL6 mice
Evaluation of different time courses of bleomycin to induced interstitial pulmonary fibrosis in C57BL6 mice
Author(s)
Abstract
Background and objectives: Animal models for human pulmonary fibrosis ideally should reflect detailed characteristics of human disease including inflammation and abnormal epithelial repair with the induction of fibrotic foci. In addition, animal models should be highly reproducible, inexpensive to maintain, easy to perform and accessible. The aim of this study to evaluate the ability of bleomycin to induce pulmonary fibrosis in animal model by different time courses and the best time for its occurrence.
Background and objectives: Animal models for human pulmonary fibrosis ideally should reflect detailed characteristics of human disease including inflammation and abnormal epithelial repair with the induction of fibrotic foci. In addition, animal models should be highly reproducible, inexpensive to maintain, easy to perform and accessible. The aim of this study to evaluate the ability of bleomycin to induce pulmonary fibrosis in animal model by different time courses and the best time for its occurrence.
Material and methods: This study was conducted in Medical Experimental Research center (MERC) in Mansoura University on C57BL/6 mice. Group (A) C57BL/6 mice (n=6) received 0.25 ml normal saline intraperitoneal twice weekly for 8 weeks and sacrificed two days after last saline dose injection. Group (B) C57BL/6 (n=10) mice received intraperitoneal injection of 0.5mg bleomycin twice weekly for 6 weeks only 6 mice completed the study and sacrificed two days after last bleomycin dose similarly. Group (C) C57BL/6 (n=10) received intraperitoneal injection of 0.5mg bleomycin twice weekly but for only 8 weeks only 6 mice completed the study and sacrificed two days after last bleomycin dose. After scarification histopathological examination of prepared slides and computerized imaging analysis were done. Weight recorded once weekly.
Results: Bleomycin induced fibrosis in the C57BL/6 mice after six and eight weeks with no significance difference between groups.
Conclusion: Bleomycin in a dose of 0.5mg for six and eight weeks induces pulmonary fibrosis in C57BL/6 mice and the six weeks model could substitute the eight weeks one.
Pages: 322-326 | 1191 Views 85 Downloads
How to cite this article:
Heba E Essa, Sayed A Abdelhafez, Mohammad E Abu Shehata, Fatma E Mostafa, Amal Fathy, Bassma Hamed, Mohamed A Sobh. Evaluation of different time courses of bleomycin to induced interstitial pulmonary fibrosis in C57BL6 mice. Int J Appl Res 2016;2(5):322-326.
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Research Journals