Vol. 3, Issue 10, Part E (2017)

Abstract
Neonatal sepsis refers to a clinical syndrome that is marked by signs and symptoms of infection in the first 28 days of life, with or without isolation of a pathogen. A retrospective study of bacterial isolates from suspected cases of neonatal septicemia was carried out over a period from Jan 2014 to Jan 2017 at the intensive care unit (ICU) of different hospitals in Akola city, Maharashtra, India. The study was carried out to determine the bacterial profile of the isolates in accordance with standard techniques and the antimicrobial susceptibility of the isolates was done by Kirby Bauer disc diffusion method. A total of 1000 blood samples for blood culture were obtained, out of which 738 were positive for bacterial isolates. Among the total 11 different types of bacterial isolates. Gram positive organisms (Staphylococcus epidermidis, Staphylococcus aureus (CONS), Streptococcus agalecticus, Aerococcus spp.) and Gram negative organisms (Klebsiella Pneumoniae, E. coli, Proteus vulgaris, Pseudomonas spp., Acinetobacter spp., Enterobacter spp. and Salmonella spp.) were the leading cause of neonatal sepsis in this study. In present investigation, gram positive isolates exhibited high sensitivity against Imipenem and Meropenem followed by Amikacin, Gentamicin and Vancomycin where as high resistance was observed against commonly used antibiotics: Penicillin, Ampicillin, Norfloxacin and Amoxycillin. Gram negative organisms had good sensitivity to Imipenem and Meropenem followed by Amikacin and Gentamicin. High resistance was noted against Penicillin, Ampicillin and Norfloxacin. It is concluded that all bacterial pathogens have emerged as the predominant pathogens responsible for neonatal sepsis which were resistant to commonly used antibiotics and multi drug resistant (MR).