Vol. 3, Issue 12, Part H (2017)
Optimization of Sclerotium rolfsii for laccase enzyme production
Optimization of Sclerotium rolfsii for laccase enzyme production
Author(s)
VR Mhaske and MS Wadikar
Abstract
In the present investigation optimization of Sclerotium rolfsii was carried out for laccase enzyme production. Different media and media amended with different nutrient sources Viz. carbon, nitrogen and sulphur and phosphorous were tested along with different physical parameters like pH, temperature and incubation period to standardized proper cultural condition for maximum laccase production. The results revealed that among the media tested malt extract broth significantly induced the laccase production as compare to other media. 6.5 pH and 30 0C temperature were optimum for laccase production where as laccase production is optimum after 8 days of incubation. Among the carbon sources tested lactose showed maximum laccase production where as nitrogen sources tested sodium nitrate showed maximum laccase production. Calcium sulphate and sodium phosphate showed maximum laccase enzyme production among the sulphur and phosphorus sources tested.
How to cite this article:
VR Mhaske, MS Wadikar. Optimization of Sclerotium rolfsii for laccase enzyme production. Int J Appl Res 2017;3(12):505-509.