Vol. 3, Special Issue 3, Part C (2017)

Abstract
Azadirachta indica A Juss. Is a very useful traditional medicinal plant in the sub-continent and each part of the tree has some medicinal properties. The present study was carried out to screen and evaluate antimicrobial activity of leaf extract of Azadirachta indica. Ethanol, Acetone and aqueous extract leaves of A. indica were tested against Escherichia coli and Staphylococcus aureus which are known to be resistant to various antibiotics. These extracts were prepared from dry Azadirachta indica leaves and evaluated for their part in increasing antibacterial activity of streptomycin against S. aureus and E. coli. The antibacterial activity of streptomycin was enhanced against the test organism in the presence of these extracts. Streptomycin in combination with these extracts showed maximum inhibition against S. aureus and E. coli. Phytochemical analysis gave positive results for steroids, triterpenoids, alkaloids, glycosides, phenolic compounds, flavonoids, and tannins. Leaf extract of Azadirachta indica contains pharmacologically bioactive constituents that may be responsible for its activity against test organisms.