Vol. 3, Special Issue 3, Part E (2017)

Abstract
The use of in vitro techniques such as anther culture, shoot organogenesis somatic embryo genesis and protoplast limitation in the application of mutation techniques in both seed and vegetatively propagated crops. In vitro culture in combination with induced mutation can speed up breeding programme, from the generation of variability through selection, to multiplication of the desired genotype. The expression of induced mutation in the pure homozygote obtained through microspore, anther or ovary culture can enhanced the rapid recovery of the desired traits.