Vol. 4, Issue 9, Part B (2018)

Abstract
The Punica granatum peel samples were collected from Panrutti District, Tamilnadu. The fruit peel were washed with water, shade dried and grinded. Powdered peel samples were individually extracted with solvents such as ethanol, methanol, acetone and water. Antibacterial activity of the extract was done by disc diffusion method. The solvent extracts (ethanol, methanol, acetone and water) of peel samples were prepared and tested against pathogens such as Escherichia coli (ATCC-8739), Staphylococcus aureus (ATCC-25923), Pseudomonas aeruginosa (ATCC-27853) and Salmonella typhimurium (ATCC-14028). Punica granatum yellow peel ethanol extract showed maximum inhibition against Staphylococcus aureus (ATCC-25923) (33mm) and Punica granatum red peel acetone extract showed maximum inhibition against Staphylococcus aureus (ATCC-25923) (30mm). GC/MS analysis was carried out for the yellow peel ethanol extract of Punica granatum in Indian Institute of Food Processing Technology (Tanjore) and seventeen major compounds were identified such as 5-Hydroxymethylfurfural, β-copaene, 7-Octadecyn 2-methyl, 17-Octadecynoic acid, n-Hexadecanoic acid, Phytol, 9,12-Octadecadienoyl chloride, Octadecanoic acid, cis-13-Eicosenoic acid, 9,12,15- Octadecatrienoic acid, 2,3-dihydroxypropyl ester, 1,2-Benzenediol, O,O'-di(2-methoxybenzoyl)-, Retinal, 9-cis-, Piperine, 6,9,12,15-Docosatetraenoic acid, methyl ester, Piperidine, 1-[5-(1, 3-benzodioxol-5-yl)-1-oxopentyl], Benzoic acid 3-methyl-4-(1, 3, 3, 3-tetrafluoro-2 methoxycarbonyl - propenylsulfanyl)-phenyl ester and β-Sitosterol.