International Journal of Applied Research
Vol. 5, Issue 12, Part D (2019)
Antifungal activity of garlic (Allium sativum) extract and honey against some selected fungi isolated from isolated from patients with skin diseases
Mathure TV, Vaidya SP, Deshpande SD and Koppikar GV
The majority of clinically used antifungal agents have various drawbacks in terms of toxicity, efficacy and cost. There are published reports available in the literature regarding antifungal activity of honey and garlic. Present study was undertaken to determine the in vitro activity of honey and garlic against various kinds of fungi isolated from patients with skin diseases, at the Department of Microbiology of T.N. Medical College and B.Y.L Nair Charitable Hospital, Mumbai. Total 510 patients were selected. Identification of Malassezia, Yeasts, Fungi and other filamentous fungi was carried out by standard methods. All Malassezia isolates were inhibited by Honey. MIC of Honey for Malassezia species ranged between 27 –30 %. All Dermatophyte isolates were inhibited by Honey. And MIC ranged from 1% v/v to 30 % v/v wherein maximum number of isolates was inhibited at 9 % v/v. All the Candida isolates were inhibited by honey and the MIC range obtained was from 3 to 27 %. Maximum numbers of isolates of Candida were inhibited by 21 % of honey. All Malassezia isolates were inhibited by Garlic extract. The MIC of garlic extract was in between 1: 25 to 1:50 dilutions. Maximum number of isolates was inhibited at 1: 25 dilution. Overall garlic extract showed 100 % in-vitro effect towards Malassezia species. On the whole, aqueous garlic extract exhibited MICs in the range of 1:25 – 1: 300 dilutions to all dermatophytes. Maximum numbers of dermatophytes were inhibited at 1:25 and 1:50 dilutions. Maximum number of Candida and yeast isolates were inhibited at MIC of 1:100 dilutions for garlic extract and the range obtained was from 1: 25 to 1: 300. The findings of this study showed that honey and extracts of garlic had a marked significance in inhibiting the test organisms. As the findings of this study compared favorably with previous studies on antifungal activity of honey and garlic, they might be a promising source of drugs for treatment of fungal infections. Further work on this study may help to design a new drug against various fungal infections.
How to cite this article:
Mathure TV, Vaidya SP, Deshpande SD, Koppikar GV. Antifungal activity of garlic (Allium sativum) extract and honey against some selected fungi isolated from isolated from patients with skin diseases. Int J Appl Res 2019;5(12):243-249.