Vol. 6, Issue 4, Part D (2020)

Abstract
The natural colour, easy production and non-toxic nature of the pigments obtained from bacteria have led them to gain worldwide significance and provided to be an alternative over its chemical counterparts. This study involves the isolation of pigment-producing bacteria from different sources like rotten vegetables, soil and air flora. Ten bacterial isolates were selected and named PR 1 to 10. The morphological characteristics and Grams nature of these isolates were studied. The isolates were identified using VITEK 2, Nucleus Diagnostic Laboratory, Kalyan. The effect of various Physical (Temperature, pH, Incubation period) and Chemical (Concentration of NaCl, Tryptone) parameters were checked for pigment production. The pigments produced were extracted with different solvents like ethanol, methanol, chloroform and acetone. The isolated pigments were characterized using Uv-Vis Spectrophotometer and TLC and were found to be Carotenoids in nature.