Vol. 10, Issue 10, Part B (2024)
Identification of phytochemicals and method development for the estimation of bioactives in polyherbal eye drops
Identification of phytochemicals and method development for the estimation of bioactives in polyherbal eye drops
Author(s)
Amartya Kumar Gupta, Rituparna Mukherjee, Nandita Das and Swapan Deb
Abstract
This study focuses on identifying the active phytochemical constituents of Polyherbal eye drops using Gas Chromatography-Mass Spectrometry (GC-MS) and developing a method for the simultaneous estimation of key Bioactives using Gas Chromatography-Flame Ionization Detection (GC-FID). Three batches of Polyherbal eye drops were analyzed. Major constituents identified include eucalyptol, phenyl ethyl alcohol, camphor, menthol, thymol, and eugenol. A GC-FID method was developed for quantifying camphor, menthol, and eugenol, showing a linearity between 10-250 µg/ml. The study provides a reliable and reproducible method for analyzing these bioactive compounds in Polyherbal eye drops with minimal sample preparation.
How to cite this article:
Amartya Kumar Gupta, Rituparna Mukherjee, Nandita Das, Swapan Deb. Identification of phytochemicals and method development for the estimation of bioactives in polyherbal eye drops. Int J Appl Res 2024;10(10):105-109.