Vol. 11, Issue 4, Part D (2025)

Abstract
Chromatography is a powerful analytical technique widely utilized for the separation and characterization of extracts or broth. This study explored and compared the chromatographic profiling of secondary metabolites from actinomycetes isolated from soil samples collected from Ikere, Ekiti State, Nigeria using Gas Chromatography-Mass Spectrometry (GC-MS) technique. Six actinomycete isolates coded DC2, LA4, LB3, LD1, SB21, and SE21 from a previous work were used for this study. The actinomycetes were grown in Starch Casein broth for 10 days at 28 ⁰C, after which were centrifuged at 4000 rpm for 30 mins. The centrifugation was repeated on the filtrate to obtain good extract, which was subjected to GC-MS analysis. Chromatographic analysis of metabolites revealed a chemically diverse range of compounds, including phenolics, fatty acids, esters, and heterocyclic structures. Notable bioactives such as phenol, (S)-(+)-1,2-propanediol, 9,12-octadecadienoic acid, and γ-sitosterol were identified, with SE21 displaying the most diverse chemical profile. These findings highlight the biosynthetic potential of actinomycetes for pharmaceutical, agricultural, and industrial applications.