Vol. 1, Issue 13, Part B (2015)

Abstract
The Continued reliance on the limited number of synthetic anthelmintics has the potential to augment widespread anthelmintic drug resistance, a serious problem already observed in many livestock production systems. The use of natural dietary compounds has the potential to be a complementary control option which may reduce this reliance on drug treatment, and slow the development of resistance. Various crude extracts from Heliotropium indicum (basic, neutral and acidic compounds) at 10 mg/mL, 50 mg/mL, 100 mg/mL and 150 mg/mL were used to evaluate the anthelmintic effect on Trichuris spp. Phytochemical analysis on the crude extract revealed the presence of plant metabolites such as Alkaloids, saponins, tannins, Reducing sugar, flavonoids and Anthraquinones. Brown crystals were obtained for all crude extracts. HPLC revealed the presence of 5,7, and 2 different isolated in the basic, acidic, and neutral crude extract from H. indicum. All extracts had effect on the motility of Trichuris spp. basic crude extracts had the lowest motility score at all concentration levels followed by Albendazole, Acid crude extract and basic crude extract within the 150 minutes of test. ANOVA performed at 0.05 significant level reveals that, there was significant reliable difference in motility above 30 minutes at all test levels both within and between groups at all the concentration level. The basic crude extract exhibited the lowest paralysis and death time at all concentrations levels with (158.4±0.59) min and (190.4±0.32) min respectively for paralysis and death at the 150 mg/mL. The acidic crude basic at the highest concentration caused paralysis and death at (406.7±0.56) min and (430.2 ±0.34) min respectively. The Neutral crude extract exhibited the highest paralysis and death time at all concentrations levels with (412.6±0.43) min and (425.3 ± 0.43) min respectively at 150 mg/mL. The extracts exhibited anthelmintic activity and thus could be an inexpensive and readily available source of Trichuris spp treatment.