International Journal of Applied Research
Vol. 2, Issue 3, Part K (2016)
Optimization studies on L-asparaginase production from endophytic Bacteria
Renuka D Joshi, Nikhilesh S Kulkarni
L-asparaginase is a well-recognized as amino acid degrading enzyme. It is recommended as therapeutic agent due to an antineoplastic activity. Hence, with the focus to optimize the enzyme production of a novel anticancer enzyme an attempt for the optimized production of L-asparaginase was done from the bacterial endophytes of selected medicinal plants. Total Twenty five endophytic bacteria were isolated from ethno medicinal plants specifically recommended for cancer therapy. Pure cultures of isolated bacterial endophytes were examined qualitatively and quantitatively, adopting spot inoculation and submerged fermentation respectively, for their optimum ability to produce L-asparaginase using M9- medium. Out of total, 25 different isolates, 3 cultures of endophytes namely Bacillus licheniformis, Bacillus pseudomycoides & Paenibacillus denitriformis showed efficient qualitatively and quantitatively enzyme activity. The optimization studies reviled that the maximum enzyme was produced at PH.8.Temp, 40 0C, & in presence of glucose as most suitable carbon source, after incubation of 48 hrs. The substrate concentration showed direct proportional relation with an enzyme activity up to 1%w/the study indicates the bacterial endophytes as a possible source of L-asparaginase as the production strain.
How to cite this article:
Renuka D Joshi, Nikhilesh S Kulkarni. Optimization studies on L-asparaginase production from endophytic Bacteria. International Journal of Applied Research. 2016; 2(3): 624-629.