International Journal of Applied Research
Vol. 3, Issue 10, Part A (2017)
Activities of some important herbal drugs and common antibiotics against human pathogens predominantly S. aureus with special reference to determination of resistant genotype by multiplex PCR assay and its correlation with herbal drugs susceptibility pattern against S. aureus
Dr. Sunit Kumar Mishra, Dr. PS Srivastava and SK Agrawal
Medicinal plant represents a rich source of antimicrobial agents. Plants are used medicinally in different countries and are a source of many potent and powerful drugs. A wide range of medicinal plant parts are used for extract as raw drugs and it’s obtaining varied medicinal properties. Several screening studies have been carried out in different parts of the world. On the antimicrobial activity of different herbal extracts. Considering the vast potentiality of plants as a source for antimicrobial drugs with reference to antibacterial and antifungal efficacy, this study was undertaken to screen the local flora for antibacterial activity. These local flora includes Acacia nilotica, Tinospora cordifolia, and Withania somnifera. The study was conducted in the Postgraduate Department of Microbiology, King George Medical University, Lucknow; from January 2011 to December 2011.The plants and pathogenic bacterium used in the research were Acacia nilotica, Tinospora cordifolia, Withania somnifera and Staphylococcus aureus respectively. The bacterium was cultured on nutrient agar and incubated at 37 °C for 24hours. The bacterium was repeatedly cultured to obtain pure isolates. Morphological and biochemical reactions were carried out to ascertain proper identification. the study shows the percentage of Staphylococcus aureus, and MRSA strains in the total specimens received. Mupirocin resistance is shown as determined by disc diffusion method, whereas methicillin resistance was determined by cefoxitin disc diffusion method. In the present study Gram-negative bacilli are the predominating pathogen (12.9%) while Gram positive cocci constituted only (4.37%). S. aureus isolates constitute only 31.4% among Gram-positive cocci while just 2.06% in overall samples received. The study shows Ashwagandha (12 ml/ml) and Babool (10mg/ml) has antimicrobial activity, but Guduchi did not show any type of antibacterial activity.
How to cite this article:
Dr. Sunit Kumar Mishra, Dr. PS Srivastava and SK Agrawal. Activities of some important herbal drugs and common antibiotics against human pathogens predominantly S. aureus with special reference to determination of resistant genotype by multiplex PCR assay and its correlation with herbal drugs susceptibility pattern against S. aureus. International Journal of Applied Research. 2017; 3(10): 18-25.