International Journal of Applied Research
Vol. 3, Issue 12, Part C (2017)
Detection of Mycobacterium tuberculosis with conventional microscopic and culture methods
Banani Jena, Rakhi Ludam, Pritam Chhotray and Mahesh Chandra Sahu
Tuberculosis is considered as the greatest cause of death worldwide. In the developing countries including Bangladesh, population density, poverty, malnutrition, and highly congested environment may create substantial risk for infection with Mycobacterium tuberculosis. The principal obstacle in the treatment of tuberculosis is the consumption of time and inaccurate diagnosis as well.
Materials and Methods: This retrospective study was carried out in order to compare results obtained by both the conventional AFB (Acid Fast Bacilli) microscopy and Lowenstein-Jensen (L-J) culture method for detection of Mycobacterium spp. in clinical samples from different categories of patients.
Results: Among one hundred and fifty samples, 83 (55.3%) AFB+ results were found under Bright-Field (BF) microscopy, 78 (52%) AFB+ and 91 (60.7%) AFB+ results were observed under conventional and Light Emitting Diode (LED) fluorescence microscopy. On L-J culture media, 103 (68.7%) AFB+ isolates were found which reveals that the culture could be a gold standard for diagnosis of TB.
Conclusion: Although AFB smear is rapid, cheap and specific test for early diagnosis of TB but its sensitivity is low and culture on LJ medium is still thought to be gold standard although takes longer time to grow and provides us with positive growth to do drug sensitivity testing.
How to cite this article:
Banani Jena, Rakhi Ludam, Pritam Chhotray and Mahesh Chandra Sahu. Detection of Mycobacterium tuberculosis with conventional microscopic and culture methods. International Journal of Applied Research. 2017; 3(12): 143-146.