Vol. 1, Issue 12, Part B (2015)

Abstract
Objective: The objective of the present work is to evaluate the presence of phytochemical constituents and antimicrobial activity of different extract of leaves of Eupatorium triplinerve Vahl. rnMethods: The serial exhaustive extraction was the clones of serial solvents: Hexane, Chloroform, Ethyl acetate and Methanol with increasing polarity using soxhlet apparatus. The Phytochemical analysis was done by using the standard procedures. Anti microbial activity was evaluated by agar diffusion method of eight human pathogens. rnResults: The results related that the leaf extracts contain a broad spectrum of secondary metabolites. Alkaloids, Flavonoids, Tannin, Saponin, Phenol, Ttriterpenes, steroids, in major proper against. Ethyl acetate extract was shown to be more effective against all the solvents followed by Hexane, Chloroform, Ethyl acetate and Methanol extracts. The Staphylococcus aureus (19.4mm-1000µg/mg) was found to be most sensitive organism followed by Streptococcus phyogens (17.8mm/1000µg/ml) and fungal strain Aspergillus fumigates (21.5mm 1000µg/ml) Aspergillus niger 19.8 mm-1000µg/ml).The stem extract of hight activity of staphylococcus aureus (19.0mm-1000µg/mg), gram negative bacteria Klebsielia pneumonia (14.2mm/1000µg/ml) and fungal pathogen Aspergillus fumigates (20.3 mm 1000µg/ml). Root extract of hight activity of staphylococcus aureus (20.8 mm-1000µg/mg), gram negative bacteria Klebsielia pneumonia (14.5mm/1000µg/ml) and fungal pathogen Aspergillus fumigates (21mm 1000µg/ml).rnConclusion: The present study concludes that the different extracts from Eupatorium triplinerve leave to contain a broad spectrum of secondary metabolites and also exhibits antimicrobial activity against all the tested microorganism. It can also be concluded that Eupatorium triplinerve plant can be exploited to discover natural products that may serve leads in the development of new pharmaceuticals.