International Journal of Applied Research
Vol. 1, Issue 12, Part G (2015)
Prevalence of various β-Lactamases production among gram negative isolates in burn care unit in a tertiary care hospital
Background: The worldwide emergence of antimicrobial resistance among a wide variety of human bacterial burn wound pathogens, particularly nosocomial isolates, limits the available therapeutic options for effective treatment of burn wound infections.rnAim: study was aimed to detect the prevalence of various types of β-Lactamases present among the gram negative bacilli isolated from burn wound infection (BWI).rnMaterials and Methods: Present study was carried out in the Department of Microbiology Dr. V M Government medical college, Solapur Maharashtra from December 2012 to July 2014.Wound swabs collected from 50 patients having total body surface area (TBSA) of burn in between 20-40% on 4th, 10th and 16th day. A total of 202 wound swabs were collected aseptically and cultured for the growth of bacteria.158 gram negative bacilli were isolated and screened for the presence of Extended Spectrum β-Lactamases (ESBL), AmpC β-Lactamases, Metallo β-Lactamases (MBL) and confirmed by the standard confirmatory tests.rnResults: 27.21% of all Gram negative isolates (158) produced extended spectrum β-lactamases, AmpC β-lactamases produced by 14.55%, 21.51% showed co-production of ESBL and AmpC and 4.43% strains produced metallo β-lactamases. Klebsiella pneumoniae was the predominant bacteria producing ESBL, AmpC and co-production of ESBL and AmpC mediated resistance, whereas Pseudomonas aeruginosa was the predominant MBL producer.rnConclusion: The emerging antimicrobial resistance in burn wound pathogens poses serious therapeutic challenge. Thus proper antibiotic policy and measures to restrict the indiscriminate use of cephalosporins and carbapenems should be taken to minimize the emergence of this multiple β-Lactamases producing pathogen.
How to cite this article:
Mundhada S, Waghmare P, Ingole k, Rathod P. Prevalence of various β-Lactamases production among gram negative isolates in burn care unit in a tertiary care hospital. Int J Appl Res 2015;1(12):474-478.