International Journal of Applied Research
Vol. 1, Issue 7, Part K (2015)
Isolation, Purification & Characterization of L-Asparaginase from dry seeds of Pisum sativum and Vigna radiata
L-Asparaginase is an extracellular enzyme having potential therapeutic application. The present work describes the isolation, extraction, purification, identification, characterization, protein estimation of powder of dry seeds of Pisum sativum and Vigna radiata. Seeds of Pisum sativum and Vigna radiata were extracted for the production of –asparaginase. L-asparaginase was assayed according to Nesselerization method based on the conversion of L-asparagine to ammonia and L-aspartate. Crude enzyme was partially purified by using dialysis technique. Maximum concentration of enzyme was found in Pisum sativum as compared to Vigna radiata. The purified enzyme was characterized for effect of temperature, pH, enzyme concentration & substrate concentration.
How to cite this article:
Kishor D. Nimkande, Zia H. Khan, Shankar M. Mular, Sangita S. Kunjwani. Isolation, Purification & Characterization of L-Asparaginase from dry seeds of Pisum sativum and Vigna radiata. Int J Appl Res 2015;1(7):628-631.