Vol. 2, Issue 12, Part A (2016)

Abstract
The aim of the present study is to analyze the effect of temperature on growth and sporulation of Aspergillus niger, Trichophyton rubrum and Rhizopus microsporus. The various temperatures employed in this investigation were 5 °C, 10 °C, 15 °C, 20 °C, 25 °C, 28 °C, 30 °C, 35 °C, 37 °C and 40 °C. Superficially cut agar discs from 8-21 days old colonies were used as inoculum. Good growth of the fungal species was observed between 25 °C and 30 °C. The fungus could not grow either at 10 °C and below or 37 °C and above. Excellent sporulation was recorded at 20 °C and 25 °C, while it exhibited good sporulation at 25 °C and 28 °C. Fair sporulation of these fungal species was observed at 30° and 35 °C. At the end of incubation period, fungal colonies were thoroughly washed and subsequently filtered on previously dried and weighed paper what man's No. 42. Filter papers containing the fungal mats were subsequently withdrawn from the oven, cooled and accurately weighed.