International Journal of Applied Research
Vol. 2, Issue 2, Part A (2016)
Activity of two indigenous Bacillus thuringiensis isolates on lepidopteran insect pest Amsacta albistriga (Arctiidae)
Two Bacillus thuringiensis isolates GM17 and GM21 were evaluated against lepidopteran insect pest Amsacta albistriga. These isolates produced various crystal inclusions with bipyramidal, spherical and cuboidal shape which confirmed by phase contrast microscopy. SDS-PAGE analysis revealed both isolates produced different Cry proteins in the range of 130 KDa to 50 KDa. Insect bioassay were carried out to determine LC50against second instar larvae of Amsacta albistriga. LC50value was found 0.055 ngml-1for Btk-HD-1 while for Bt isolates GM17 and GM21, LC50values were found 0.017 ngml-1 and 0.027 ngml-1 respectively. Phylogenetic analysis of cry1Acgene sequence of GM17 isolate was found point mutation in domain II which can be used for domain swapping and cry2 gene of GM21 to gene pyramiding strategies for insect resistance management.
How to cite this article:
Jigar V Shah, Shailesh B Lad, Rakeshkumar Yadav, Sanjay S Ingle. Activity of two indigenous Bacillus thuringiensis isolates on lepidopteran insect pest Amsacta albistriga (Arctiidae). Int J Appl Res 2016;2(2):20-23.