Vol. 2, Issue 2, Part F (2016)

Abstract
The present work aimed to estimate the dietary supplement of mixture of medicinal plant leaf extract of Solanum trilobatum and O.sanctum as well as the individual effect on immune response of fresh water fish Mystus keletius. Non-specific immune assays such as phagocytic activity, serum antiprotease activity and serum myeloperoxidase activity were analysed in freshwater cat fish Mystus keletius. The blood sample was collected for every seven days after the final feeding. Levels of phagocytic activity, serum antiprotease activity and serum myeloperoxidase activity were increased in the group of fish fed with supplemented diet than the group fed with control diet. This increase was based on the concentration of plant extracts mixture and duration of supplemented diet. This increase was more in fish supplemented with Solanum trilobatum leaf extract than in fish treated with Ocimum sanctum leaf extract but lesser than the fish fed with mixture of aqueous leaf extracts of both in 1:1 ratio. Highest level of phagocytic activity, serum antiprotease activity and serum myeloperoxidase activity were confirmed when the fish fed with 1% of mixture of aqueous extracts of Solanum trilobatum and O.sanctum in the 4^th week of the experiment. The synergistic effect of medicinal plant aqueous leaf extracts mixture on immunostimulatory activity of fish is highlighted.