International Journal of Applied Research
Vol. 2, Issue 2, Part I (2016)
Fractionation, radical scavenging activity of bioactive compounds and FT-IR spectroscopy study of milky mushroom, Calocybe indica Var. APK2
Antioxidant activity of solvent fractions of Calocybe indica Var.APK2 was assessed by methanolic extraction. The chloroform and ethanol fractions of C. indica were evaluated via hydroxyl radical scavenging assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Six fractions (F1 to F6) were obtained from chloroform-ethanol partition using silica gel chromatography. Among the six fractions, fraction -3 and fraction -4 recorded high antioxidant properties. In DPPH radical scavenging activity, 1ml concentration of fraction 3 expressed 50.19% of EC50 value, where as in Hydroxyl radical scavenging activity, 0.8ml concentration of fraction 4 expressed 50% of EC50 value, which was compared with the standards namely L-Ascorbic acid and Butylated Hydroxy Anisole (63.33 and 69.19 respectively). The FTIR analysis of methanol extracts of Calocybe indica var APK.2, confirmed the presence of alcohols, phenols, alkanes, aldehydes, saturated aliphatic, unsaturated ketones, primary amines, alkanes, aromatics, alkyl halides and aliphatic amines, with their major peaks.
How to cite this article:
A Parveen Nisha, R Kumuthakalavalli. Fractionation, radical scavenging activity of bioactive compounds and FT-IR spectroscopy study of milky mushroom, Calocybe indica Var. APK2. Int J Appl Res 2016;2(2):565-568.