Vol. 2, Issue 2, Part J (2016)

Abstract
Callogenic response of various parts of explant such as leaf, internode, node, shoot tip and cotyledons of Momordica tuberosa was tried on MS medium with varied concentrations of auxins (NAA and 2,4-D) and cytokinins (BA, IBA, BAP). The green compact callus was produced in all the explants in all the combinations except cotyledons. Maximum fresh weight (580±8.82 mg) was observed in the callus derived from internodes on MS+0.8 mg/l BA+0.8 mg/l 2, 4-D). Earlier response (7 days) was observed in the leaf and shoot tips on MS+0.9 mg/l IBA+0.4 mg/l NAA. Cotyledons showed delayed (21 days) response on MS+BAP+2, 4-D, where the callus was white compact which turned green.