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International Journal of Applied Research
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ISSN Print: 2394-7500, ISSN Online: 2394-5869, CODEN: IJARPF

Impact Factor: RJIF 8.4

International Journal of Applied Research

Vol. 2, Issue 5, Part N (2016)

A comparison study of carbol fuchsin and papanicolaou staining methods for the demonstration and enumeration of barr bodies in buccal smear

Author(s)
Dr. Narendra Kumar Meena, Dr. Mohan Singh and Dr. Jaskaran Singh
Abstract
Introduction: Barr body is of the X chromosomes formed from random inactivation and condensation of one of the two female chromosomes in virtually all the somatic cells of female mammals. In practice stains such as Papanicolaou, and carbol fuchsin, have proved suitable because they produced stronger contrast and are much simple to manipulate.
Aim: Our study aimed to assess the efficacy of gender determination by a comparison between Carbol fuchsin and Papanicolaou stain in buccal mucosal scrapings.
Material and Methods: For the study to be conducted, 500 samples were taken in total, with 250 smears for carbol fuchsin stain and 250 smears for papanicolaou stain obtained from students. The smears collected were subsequently stained with carbol fuchsin and papanicolaou stains.
Results and Discussion: In considering descending order of merit, papanicolaou stain yielded 46.14% of barr body positive cells, against 8.68% of barr body positive cells harvested by carbol fuchsin stain. Papanicolaou stain excelled in both the efficacy and accuracy, from the carbol fuchsin stain.
Conclusion: In comparison the papanicolaou stain came out better and low time consuming as compare to the carbol fuchsin stain.
Pages: 941-944  |  687 Views  27 Downloads
How to cite this article:
Dr. Narendra Kumar Meena, Dr. Mohan Singh, Dr. Jaskaran Singh. A comparison study of carbol fuchsin and papanicolaou staining methods for the demonstration and enumeration of barr bodies in buccal smear. Int J Appl Res 2016;2(5):941-944.
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International Journal of Applied Research