International Journal of Applied Research
Vol. 2, Issue 7, Part H (2016)
Quantitative estimation of amino acids and carbohydrates present in the root exudates of Suaeda nudiflora and Cassia auriculata
Substances released by healthy and intact roots into the environment are collectively designated as root exudates. In present study, Suaeda nudiflora Moq. And Cassia auriculata Linn. were selected as experimental plants. S. nudiflora, a halophytic species; it has the potential as a future oil seed crop and is highly suitable for producing high protein biomass while C. auriculata is used as fodder for camels and goats and also known for its various medicinal properties like anthelminthic, antidiabetic, etc. Root exudates were collected from both the plants and carbohydrate and amino acids were measured quantitatively. Protein/amino acids estimation were done by the methods Ninhydrin and Bradford’s colorimetric assay while carbohydrate estimation was done by the methods Anthrone and Phenol sulfuric Acid colorimetric assay. Result of this study showed that C. auriculata was showing better result than S. nudiflora in Ninhydrin, Bradford’s and Phenol-sulfuric Acid colorimetric assays while S. nudiflora was showing better result than C. auriculata in Anthrone colorimetric assay. In the rhizosphere, root exudate plays an important role as attracting signal molecules to attract the plant growth promoting microbes (endophytes and rhizobacteria) which performs important plant growth promoting functions (biocontrol, hormone production and bioremediation). Experimental plants showed the presence carbohydrate and amino acids in root exudates, which will help them in the promotion of plant growth, immunity and increase the resistance to arid and saline conditions which is not only of economic importance but also counted as contribution to the agriculture field.
How to cite this article:
Mahavi O Makadia, Nilesh S Panchal. Quantitative estimation of amino acids and carbohydrates present in the root exudates of Suaeda nudiflora and Cassia auriculata. Int J Appl Res 2016;2(7):542-548.