Vol. 3, Issue 3, Part K (2017)

Abstract
The most alternative plastic waste treatment method is enzymatic degradation. These kind of depolymerising enzymes are effectively produced by some microorganisms. This study revealed that, microorganisms produce depolymerising enzymes during biodegradation of polyethylene carry bag (PCB). For that, in sterile minimal broth, pure culture of various isolates were added, pieces of mass polyethylene carry bag films were added and incubated for a month. After the incubation the broth was centrifuged at 10,000 rpm for 20 minutes at 4 °C. The bacteria Pseudomonas aeruginosa, Bacillus sp and fungi Fusarium graminearum were found producing depolymerising enzymes viz., amylase, lignin /manganese peroxidase and laccase followed by others. These potential bacteria and fungi might be applied for bioremediation in the polyethylene-contaminated environments.