Vol. 3, Special Issue 3, Part E (2017)

Abstract
Plants have been used traditionally in local people for treatment of bone fracture. The plant parts (extract) identified e.g. (root) serve as major source of active ingredient and products of secondary metabolites e.g alkaloid, terpenoids etc. used in curing diseases. The phytochemical analysis of Vitex negundo L was evaluated to ascertain some of the secondary metabolites that exhibit medicinal properties. The results of phytochemical screening of ethanol crude root extract of Vitex negundo Lrevealed the presence of alkaloids, tannins, saponins and flavonoids. These metabolites observed by various techniques like solvent extraction by ultra-sonicator, rota-vapour, thin layer chromatography column separation and HPTLC technique