Vol. 4, Issue 2, Part C (2018)

Materials and methods: Thirty patients were selected based on inclusion and exclusion criteria. After rubber dam isolation access opening was done and collection of sample using absorbent paper point was done. Samples were processed for microbiological procedure and isolation of different species of bacteria was done. All the individual species were subjected to antibacterial sensitivity for three irrigants.

Results: Different species of obligatory and facultative anaerobes were isolated mainly Peptostreptococcus colonies of obligatory anaerobic gram positive cocci followed by facultative anaerobe E. faecalis, followed by gram negative Bacilli such as P. intermedia, Porphyromonas species, Bacteroides species, and Fusobacterium species. Facultative Gram-positive anaerobic cocci such as Streptococcus pyogenes, S. sobrinus, and Staphylococcus aureus were also found but were comparatively less in number. These were subjected to antibacterial sensitivity against three irrigants. The results statistically analysed using Pearson’s Chi-square test for two non-parametric data and proportional comparisons were done using Z test for two sample proportion. Chlorhexidine was found most sensitive, followed by Fennel extract and least sensitive is Fenugreek Extract for facultative as well as obligatory anaerobes.

Conclusion: The bacterial profile in infected primary teeth consists of mainly obligatory anaerobes Peptostreptococcus colonies, followed by E. Faecalis and black pigmented colonies. Amongst two herbal irrigant, fennel can act a potent herbal substitute for chlorhexidine as intracanal irrigant in infected primary teeth.