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International Journal of Applied Research
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ISSN Print: 2394-7500, ISSN Online: 2394-5869, CODEN: IJARPF

IMPACT FACTOR (RJIF): 8.4

Vol. 4, Issue 2, Part D (2018)

Effect of growth regulators on callus and multiple shoot formation of Datura metel L

Effect of growth regulators on callus and multiple shoot formation of Datura metel L

Author(s)
Sallykutty Thomas
Abstract
Effect of growth regulators on callus and multiple shoot formation was studied in Datura metel L. The terminal bud and nodal segments were cultured in MS basal medium containing four different growth regulators such as 2,4-D, Kinetin, IAA and BA. MS medium supplemented with combinations of 2mg/l kinetin and 1mg/l IAA showed profuse callus formation. Nodal segments also showed profuse callus formation when MS medium was supplemented with combinations of 1.5mg/l Kinetin and 2mg/l BA. When combinations of 1mg/l Kinetin and 2mg/l BA were given only nodal segments showed multiple shoot formation.
Pages: 216-219  |  967 Views  86 Downloads


International Journal of Applied Research
How to cite this article:
Sallykutty Thomas. Effect of growth regulators on callus and multiple shoot formation of Datura metel L. Int J Appl Res 2018;4(2):216-219.
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