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ISSN Print: 2394-7500, ISSN Online: 2394-5869, CODEN: IJARPF

IMPACT FACTOR (RJIF): 8.4

International Journal of Applied Research

Vol. 5, Issue 10, Part E (2019)

Impact of ethyl alcohol on the dinitrosalicylic acid assay for reducing sugars

Author(s)
Dr. Aradhana Verma
Abstract
The determination of reducing sugars, especially glucose, is of considerable interest. An assay attributed to Miller using 3, 5-dinitrosalicyclic acid (DNS) is widely used but suffers from some limitations. It is, nonetheless, convenient and recommended by the IUPAC Commission on Biotechnology for measuring cellulase activity. We found that 5-10% concentrations of methanol, ethanol, 1- and 2-propanol interfered with the DNS assay and gave a positive error (an apparent increase in glucose) when aqueous standards were used for calibrating the assay. For ethanol concentrations beyond about 15%, the assay gave a negative error. In the case of ethanol, this was due to an effect of ethanol on the color- forming reaction as well as a slight effect on the spectral absorbance of the product.
Pages: 368-371  |  24 Views  0 Downloads
How to cite this article:
Dr. Aradhana Verma. Impact of ethyl alcohol on the dinitrosalicylic acid assay for reducing sugars. Int J Appl Res 2019;5(10):368-371.
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