Vol. 8, Issue 4, Part E (2022)

Abstract
The study focuses on structural genomics-related parallel screening studies on the impact of fusion tags (particularly the His tag) on protein solubility and the use of fusion tags in quick, parallel purification protocols designed for preliminary biophysical analysis of human proteins produced in E. coli. It was discovered that the His tag had a detrimental effect on protein solubility for the majority of proteins. For our C-terminal His tag compared to the N-terminal His tags employed in this work, this influence seems to be more apparent. Furthermore, different vector - target protein combinations lead to significant variances in host cell growth rates, so high ratios of soluble to total protein may not always guarantee a high yield of soluble protein after purification. It has been able to express, purify, and analyze the structural properties of low concentration samples of 15N-labeled proteins in one or two days thanks to the development of protein purification techniques for various fusion tags.