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International Journal of Applied Research
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ISSN Print: 2394-7500, ISSN Online: 2394-5869, CODEN: IJARPF

IMPACT FACTOR (RJIF): 8.4

Vol. 8, Issue 5, Part B (2022)

Method development and validation of simultaneous determination of Eugenol, Thymoquinone and β-Careophyllene in mixed spice samples

Method development and validation of simultaneous determination of Eugenol, Thymoquinone and β-Careophyllene in mixed spice samples

Author(s)
Rituparna Mukherjee, Amartya Kumar Gupta and Nandita Das
Abstract
A rapid and simple Gas Chromatographic (GC) method has been developed for the simultaneous quantification of eugenol, thymoquinone and β-careophyllene in individual and mixed spice samples. Analysis was performed using capillary column (30 m x 0.25 mm x 0.25 µm) by using Flame ionization detector. Mixed spices were extracted by methanol. The calibration plot was linear over the range studied (2-500 ng for each eugenol, thymoquinone and β-careophyllene standards) with a correlation of 0.99 for eugenol, thymoquinone and β-careophyllene. The method was also validated for the linearity, range, precision, recovery and detection limits. Thus, the method is suitable for routine analysis of eugenol, thymoquinone and β-careophyllene in mixed spices.
Pages: 92-97  |  486 Views  99 Downloads


International Journal of Applied Research
How to cite this article:
Rituparna Mukherjee, Amartya Kumar Gupta, Nandita Das. Method development and validation of simultaneous determination of Eugenol, Thymoquinone and β-Careophyllene in mixed spice samples. Int J Appl Res 2022;8(5):92-97. DOI: 10.22271/allresearch.2022.v8.i5b.9725
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