Vol. 9, Issue 4, Part B (2023)

Abstract
The inclusion of mustard oil in the Indian diet is due to its numerous health benefits, but its nutritional value is limited by the high levels of erucic acid. To address this issue and impart a sweet flavor, coriander seeds were added to create Sweet Mustard Oil. The objective of this study was to produce Sweet Mustard Oil and identify its bioactive chemical constituents using GC-MS analysis through Trace 1300 series gas chromatography (Thermo scientific). The experimental conditions of the GC-MS system involved a run time of 48.36 minutes, sample injection volume of 1 ml, a maximum temperature of 350 °C, and mass spectral scan range of 40 to 500 atomic mass units. Data evaluation of GC-MS chromatogram was performed using GC/MSD Chem Station software. The separated compounds were named and their molecular weight and structure were determined by comparing the mass spectra with data from National Institute of Standards and Technology (NIST) libraries. The GC-MS analysis of Sweet Mustard Oil showed the presence of 50 different compounds, with the major compounds being Benzoic acid, 4-ethoxy, ethyl ester (24.88%), Alpha phenyl alpha tropylacetaldehyde tosylhydrazone (8.82%), çSitosterol (6.74%), Glycerol 1palmitate (4.70%), and 9,12 Octadecadienoicacid (Z,Z), 2-hydroxy1(hydroxymethyl) ethyl ester (13.24%). Other compounds identified included various alkanes, alcohols, esters, and acids. This information provides insight into the composition of Sweet Mustard Oil and its potential applications in various industries.